Neuroimmune Circuits and Infectious Disease
Emory University - Atlanta, Georgia
March 21-25, 2001

Program:

Wednesday; March 21, 2001

5:00-6:30PM SNIP Meeting for Counselors and Committee Members, Emory Conference Center, Billiard Room Bar

7:00-9:30 Dinner, SNIP Counselors and Committee Members

Thursday; March 22, 2001

9:00AM-Noon Meetings of SNIP committees, Emory West, Room 154 & Breakout Rooms

Noon-1:30PM Luncheon, Counselors and Committee Members, Emory West

2:00-4:30PM Meeting of SNIP Board of Counselors, Emory West, Room 154

5:00-7:00 Opening Reception and Poster Session Emory Convention Center Hotel, Salon I
Open Bar and Hors d'oveure (5:00-6:00 PM)

Poster Presentations (set-up after 4:00 PM; manned from 5:00-7:00 PM):
1. Morphine induced apoptosis in endothelial cells: FAS/FAS-L dependent? Jenine K . Anday and S.L. Chang, Seton Hall University
2. Role of apoptotic macrophages in morphine induced bacteremia and sepsis. Madhu C. Bhaskaran, R. Neetu, K. Reddy, J. Singh, G. Patel, J. Ahmed and P.C. Singhal, Long Island Jewish Medical Center
3. Opiate effects on HIV/HTLV infections. Chuanhai Cao, S.B. Nyland, Y. Bai, S. Specter and K.E. Ugen, University of South Florida
4. Cytomegalovirus induces cytokine and chemokine production differentially in microglia and astrocytes: antiviral implications in the CNS. Maxim C-J. Cheeran, S. Hu, S.L. Yager, G. Gekker, P.K. Peterson and J.R. Lokensgard, Minneapolis Medical Research Foundation and the University of Minnesota Medical School
5. Neuromodulation of Salmonella choleraesuis entry into absorptive epithelia and follicle-associated epithelia of porcine jejunum. Benedict T. Green and David R. Brown, University of Minnesota
6. Morphine modulates HIV-1 GP160-induced macrophage apoptosis. Aditi A. Kapasi, G. Patel, K. Jain, M. Pandya and P.C. Singhal, Long Island Jewish Medical Center
7. Morphine modulates FAS/FAS ligand expression in porcine glial cells. Jinhee Lee, M.R. Olin, K.H. Choi and Thomas W. Molitor, University of Minnesota
8. Methadone enhances HIV replication in human immune cells. Yuan Li, X. Wang, C.-J. Guo, S.D. Douglas and W.-Z. Ho, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine
9. Expression of mu opioid receptors in human HL-60 promyelocytic leukemic cells. Xin Mao, J.A. Patel, W.X. Li and S.L. Chang, Seton Hall University
10. The immunosuppressive effect of morphine on swine gamma-delta T cells in BCG vaccinated pigs. Michael R. Olin, J.H. Lee, K.H. Choi and T.W. Molitor, University of Minnesota
11. Opioids, HIV-1, GP120 and TAT modulate hemoxygenase expression in human monocytes. Radhakrishnan Neetu, M. Baskaran, n. Franki, K. Reddy, A. Kapasi and P.C. Singhal, Long Island Jewish Medical Center
12. Immune mechanisms mediating immunosuppression following withdrawal from morphine in mice. Rahil T. Rahim, J.J. Meissler, A. Cowan, T.J. Rogers, E. B. Geller, M. W. Adler and T. K. Eisenstein, Temple University
13. Role of opioid receptors in mediating neurotoxicity caused by morphine and HIV proteins. Jadwiga Turchan, D. Galey, K.F.Hauser and A. Nath, University of Kentucky
14. Morphine modulates lymph node lymphocyte function through a mechanism involving Caspase-3, Caspase-8, nitric oxide and apoptosis. Jinghua Wang, R. Charboneau, R. A. Barke, H.H. Loh and Sabita Roy, University of Minnesota and the VA Medical Center
15. Substance-P and histamine phase shift the circadian clock through activation of
glutamate receptor-mediated nitric oxide pathway. Jian M. Ding, University of Minnesota
16. Naloxone potentiates anti-HIV-1 activity of antiretroviral drugs in CD4+ lymphocyte cultures. Genya Gekker, J. Lokensgard, S. Hu and P.K. Peterson, University of Minnesota
17. Morphine-induced apoptosis in human brain cell cultures. Shuxian Hu, W.S. Sheng, J.R. Lokensgard and P.K. Peterson, Minneapolis Medical Research Foundation and the University of Minnesota
18. HIV-1 GP160 modulates morphine-induced macrophage apoptosis: role of nitric oxide. Aditi A. Kapasi, G. Patel, J. Singh, K. Reddy and P.C. Singhal, Long Island Jewish Medical Center
19. Renal tubular cell CD4 receptor and HIV-1 GP120 interaction with P300 modulates human renal tubular epithelial cell growth by activation of AP-1 complex and NF-kB. Aditi A. Kapasi, S. Fan, N. Franki and P.C. Singhal, Long Island Jewish Medical Center and Albert Einstein College of Medicine
20. The role of endogenous opioids in moderate exercise-training induced enhancement of secondary antibody response. Zoher F. Kapasi, P.A. Catlin, J. Beck, T. Kelley and K. Smith, Emory University
21. Mu-opioid induction of RANTES, IP-10 and MCP-1 production: role of NFkB and cytokines. Michele A. Kutzler, E.E. Henderson, T.K. Eisenstein, M.W. Adler and T. J. Rogers, Temple University School of Medicine
22. Substance P antagonist (CP-96,345) inhibits HIV-1 replication in human mononuclear phagocytes. Jian-Ping Lai, W.-Z. Ho, X. Zhan, Y. Yi, R.G. Coleman and S.D. Douglas, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine
23. Diazepam inhibits TAT-induced migration and intracellular calcium mobilization in human microglia. James R. Lokensgard, S. Hu, C.C. Hegg, S.A. Thayer, G. Gekker and P.K. Peterson, M.M.R.F., University of Minnesota
24. A primate model of HIV infection: neuron loss and neuropathology in the basal ganglia. Joanne K. Marcario, K.F. Manaye, P.R. Mouton, K. SantaCruz, N.E.J. Berman and P. Cheney, University of Kansas Medical Center, Howard University and Johns Hopkins School of Medicine
25. Expression of authentic substance P receptors in murine and human dendritic cells. Ian Marriott and K.L. Bost, University of North Carolina, Charlotte
26. Opiate-dependent chick embryos also exposed in ovo to naloxone show altered fever responses to LPS post-natally. Lisa M. Schrott and Sheldon B. Sparber, University of Minnesota
27. U50,488 inhibits HIV-1 TAT-induced MCP-1 production by human astrocytes. Wen S. Sheng, S. Hu, J.R. Lokensgard and P.K. Peterson, Minneapolis Medical Research Foundation and the University of Minnesota
28. Opioid administration modulates HIV-1 coreceptor expression and HIV-1 replication. Amber D. Steele, T.K. Eisenstein, M.W. Adler, E.E. Henderson and T. J. Rogers, Temple University School of Medicine
29. The effects of cocaine, norepinephrine and HIV infection on leukocyte endothelial cell interactions and cardiovascular disease. Bruce Sundstrom, D.E. Martinson, R.M. Donahoe and A.A. Ansari, Emory University
30. Opioids and Keratinocytes: neuroectodermal heritage versus immunoregulatory capacity. Imre Szabo, A. Cowan and T. J. Rogers, Temple University School of Medicine
31. Pharmacological characterization of the opioid receptor(s) modulating Fc phagocytosis in murine peritoneal macrophages. Nilka I. Tomassini, F.L. Renaud, S. Roy and H.H. Loh, Universities of Puerto Rico and Minnesota
32. A modified ELISA assay for testing monkey anti-morphine antibody. Yichong Wang, P. Langford-Turner, J.J. Madden, D. Anderson, S. O'Neil, H. McClure, F. Novembre, L. Howell, G. Niedziela, S. Ehnert and R.M. Donahoe, Emory University and Yerkes Regional Primate Research Center
33. Neuroendocrine, immune and somatic alterations in rats physically dependent on heroin. Richard J. Weber, R. Gomez-Flores, J. E. Smith and T.J. Martin, University of Illinois College of Medicine, Peoria, Universidad Autonoma de Nuevo Leon and Wake Forest University

Friday Morning - March 23, 2001

7:30-8:15 AM Technology School -Oligonucleotide array technology and applications, including studies in cancer classification, muscular distrophy, aging and obesity. Mark Hurt, Affymetric, Inc. Grace Crum Rollins School of Public Health, Room P45

8:15-9:00 Continental Breakfast

9:00-Noon Symposium 1 - Opioid and cannabinoid receptors on immune cells Jean M. Bidlack, University of Rochester & Claire Gaveriaux-Ruff, C.N.R.S.

9:00-9:45 Plenary Lecture - Studies on the expression and function of delta opioid receptors by T-cells. B. Sharp, Tennessee

9:45-10:00 Implication of opioid receptors in immune responses: Studies with MOR, DOR and KOR knockout mice. Claire Gaveriaux-Ruff, C.N.R.S.

10:00-10:15 Mu opioid receptor potentiates LPS-induced cytokine secretion in HL-60 cells. Sulie L. Chang, Seton Hall University

10:15-10:35 Coffee Break

10:35-10:50 The use of fluorescein-conjugated opioids to label delta and mu opioid receptors on lymphocytes. Jean M. Bidlack, University of Rochester

10:50-11:05 Expression of cannabinoid receptors on rat microglia. Guy A. Cabral, Virginia Commonwealth University

11:05-11:20 Increase in CB1 and CB2 mRNA expression in PBMC's from Marijuana smokers. Liang Nong, South Florida

11:20-Noon Discussion of topic by lecturers and attendees

Noon-2:00PM Lunch on own

Friday Afternoon - March 23, 2001

2:00-5:00 Symposium 2 - Interactions between opioid/cannabinoid receptors with chemokine ligands/receptors Guy A. Cabral, Virginia Commonwealth University & Sulie L. Chang, Seton Hall University, Grace Crum Rollins Building, Rita Ann Rollins Lecture Hall, 8th Floor, Room 860

2:00-2:45 Plenary Lecture - Cross-regulation of G protein-coupled receptors and their ligands Thomas J. Rogers, Temple University School of Medicine

2:45-3:05 Evidence for the involvement of altered intracellular calcium in cannabinoid-mediated modulation of interleukin (IL)-2 and IL-4. Norbert E. Kaminski, Michigan State University

3:05-3:25 Polymorphism of CCR5. Ronald Y. Chuang, University of California, Davis

3:25-3:45 Cross-desensitization between the mu opioid receptor and the chemokine receptor CCR5. Lee-Yuan Liu-Chen, Temple University School of Medicine

3:45- 4:00 Coffee Break

4:00-4:20 Morphine potently inhibits B-chemokine production and chemotaxis of human immune cells. Wen-Zhe Ho, Children's Hospital of Philadelphia

4:20-4:40 Effects of opioids on chemokine gene regulation. Supriya Mahajan, SUNY, Buffalo

4:40-5:00 Opioid receptor ligand suppression of HIV-1: Potential involvement of chemokine receptors. James R. Lokensgard, Minneapolis Medical Research Foundation and the University of Minnesota Medical School

5:00-5:20 Discussion of topic by lecturers and attendees

Dinner on own

Saturday Morning - March 24, 2001

7:30-8:15 AM Technology School - The T cell immunology tool kit. Mark Edinger, Pharmingen, Inc.; (Grace Crum Rollins Building, Rita Anne Rollins Lecture Hall, Room 860)

8:15-9:00 Continental Breakfast

9:00-Noon Symposium 3 - Effects of drugs of abuse on Th1/Th2 polarization and other cell functions Toby K. Eisenstein, Temple University School of Medicine & Herman Friedman, University of South Florida School of Medicine

9:00-9:35 Plenary Lecture - Beta-2-adrenergic receptor expression and function on Th1 and Th2 cells. Virginia Sanders, Loyola University Medical Center

9:35-10:10 Endogenous opioids and opiate drugs differentially regulate T-cell and macrophage cytokines. Paola Sacerdote, University of Milan

10:10-10:25 Coffee Break

10:25-10:45 Morphine treatment in vitro redirects T-cells toward Th2 differentiation. Sabita Roy, University of Minnesota

10:45-11:05 Methionine-enkephalin modulates T-cell cytokine polarization. Bonnie Miller, Southwestern Medical Center

11:05-11:25 Cannabinoid effects on GATA-3 and T-helper cells. Thomas Klein, University of South Florida Medical School

11:25-11:45 Cannabinoid modulation of human T-helper cells. Michael Roth, UCLA School of Medicine

11:45-Noon Discussion of topic by lecturers and attendees

Noon-2:00 SNIP Luncheon, Reception room, Rita Anne Rollins Lecture Hall
SNIP General Membership Meeting, Rita Anne Rollins Lecture Hall
All conferees are invited to attend, eat lunch and participate in discussing your future and the future of SNIP.

Saturday Afternoon - March 24, 2001

2:00-5:00 Symposium 4 - The immune system as target for drugs of abuse and HIV infection
Jag Khalsa, NIDA & Richard Weber, University of Illinois, Peoria

2:00-2:45 Plenary Lecture - Interactions between HIV and the immune system. Mark Feinberg, Emory University CFAR

2:45-3:05 Chronic opioid treatment and abstinence- induced opioid immune alterations: relevance to HIV progression. Toby K. Eisenstein, Temple University School of Medicine

3:05-3:20 Fever, cytokines and SIV infection in the rhesus macaque. Lisa J. Madden, Scripps Institute

3:20-3:40 Immunological & virological effects of morphine-dependency on early-stage SIV infection in rhesus macaques. Robert M. Donahoe, Emory University

3:40-4:00 Coffee Break

4:00-4:20 Correlation of immunological and virologic parameters with encephalitis during acute SIV infection. Shawn P. O'Neil, Emory University

4:20-4:40 Neuroimaging studies in HIV and methamphetamine abuse. Linda Chang, Brookhaven National Labs

4:40-5:00 Discussion of topic by lecturers and attendees

6:30-9:30 Awards Banquet - Thomas Klein, John J. Madden and Burt Sharp, moderators
Lecture - Control of mitochondrial function by diazepam. Douglas C. Wallace, Emory University
Emory Conference Center Hotel, Salon 1

Sunday Morning - March 25, 2001

8:30-9:00AM Continental Breakfast, Grace Crum Rollins Building, Rita Anne Rollins Lecture Hall, Room 860

9:00-Noon Symposium 5 -Drugs of abuse and HIV infection
Avindra Nath, University of Kentucky & Walter Royal, III, Morehouse School of Medicine;

9:00-9:30 Plenary Lecture - Neural Epidemiology of HIV infection. Walter Royal, III, Morehouse School of Medicine

9:30-10:00 HIV, psychoactive substance use and cognitive-motor disorder: A synergism?. Karl Goodkin, University of Miami School of Medicine

10:00-10:30 Therapeutic approaches in drug abusers with HIV infection. Avindra Nath, University of Kentucky

10:25-10:40 Coffee Break

10:40-10:55 Deranged immune status following alcohol ingestion. Pravin C. Singhal, Long Island Jewish Medical Center

10:55-11:10 Synergistic effects of heroin and HIV gp120 on ICAM-1 and nitric oxide production by brain microvascular endothelial cells (BMVEC). Madhavan P.N.Nair, SUNY, Buffalo

11:10-11:30 Opiates and HIV-TAT protein synergistically destabilize neuronal function in vivo. Kurt F. Hauser, U. of Kentucky

11:30-Noon Discussion of area by lecturers and attendees

Adjournment.